We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Nystatin-enhanced glutathione production by S accharomyces cerevisiae depends on γ-glutamylcysteine synthase activity and K<sup>+</sup>.
- Authors
Zhao, Yucheng; Bian, Xiaohong; You, Xiaoqing; Shao, Fei; Xiang, Xi; Deng, Xuepeng; Zhao, Ganggang; Xu, Jiyang
- Abstract
Glutathione ( GSH), an important tripeptide compound, is widely used as a therapeutic and in the food and cosmetic industries. To improve its production yield, we added the antibiotic nystatin to a batch fermentation of S accharomyces cerevisiae, at different concentrations and at various times. Based on the results that nystatin can effectively stimulate GSH accumulation but at the same time inhibits cell growth, a three-point addition strategy (0.05 mg/L at 8 h, 0.25 mg/L at 16 h, and 0.5 mg/L at 20 h) was developed to maximize GSH production. As a result, a maximum yield of 237.8 mg/L was obtained, which was by 50.6% higher than without the addition of nystatin. When combining this strategy with cysteine addition, the GSH yield increased to 278.9 mg/L. Subsequently, the γ-glutamylcysteine synthetase (γ- GCS) activity and K+ concentration were analyzed to investigate the possible mechanism involved in the increased production. It was found that the nystatin-induced increase in the GSH yield was associated with a higher γ- GCS activity and K+ concentration.
- Subjects
GLUTATHIONE; NYSTATIN; SACCHAROMYCES cerevisiae; FERMENTATION; CELL growth; CYSTEINE; POTASSIUM ions
- Publication
Engineering in Life Sciences, 2013, Vol 13, Issue 2, p156
- ISSN
1618-0240
- Publication type
Article
- DOI
10.1002/elsc.201200055