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- Title
Nucleocapsid gene variability reveals two subgroups of Lettuce necrotic yellows virus.
- Authors
Callaghan, B.; Dietzgen, R. G.
- Abstract
The complete nucleocapsid (N) genes of eight Australian isolates of Lettuce necrotic yellows virus (LNYV) were amplified by reverse transcription PCR, cloned and sequenced. Phylogenetic analyses of these sequences revealed two distinct subgroups of LNYV isolates. Nucleotide sequences within each subgroup were more than 96% identical but heterogeneity between groups was about 20% at the nucleotide sequence level. However, less than 4% heterogeneity was noted at the amino acid level, indicating mostly third nucleotide position changes and a strong conservation for N protein function. There was no obvious geographical or temporal separation of the subgroups in Australia.
- Subjects
AUSTRALIA; LETTUCE diseases &; pests; NUCLEOTIDE sequence; GENETIC transcription; GENETIC code
- Publication
Archives of Virology, 2005, Vol 150, Issue 8, p1661
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-005-0528-7