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- Title
Citrobacter diversus ULA27 produces two forms of a chromosomal beta-lactamase.
- Authors
Oliva, Brunello; Marinucci, Maria Contina; Amicosante, Gianfranco; Oratore, Arduino; Bennett, Peter M.; Oliva, B; Marinucci, M C; Amicosante, G; Oratore, A; Bennett, P M
- Abstract
A multi-resistant clinical isolate of Citrobacter diversus, particularly resistant to penicillins and cephalosporins, has been studied. The strain produced constitutively high levels of beta-lactamase which efficiently hydrolysed cephaloridine but not ampicillin. The apparent Km value for cephaloridine (125 +/- 25 microM) was within the range usually observed for beta-lactamases in similar bacteria. The strain possessed two forms of the enzyme as indicated by isoelectric focusing patterns, HPLC analysis and two pH optima. SDS-PAGE analysis indicated a single protein responsible for both forms. The strain possessed a single plasmid, as revealed by agarose gel electrophoresis, but beta-lactamase production was not plasmid encoded. The available data indicate the beta-lactamase(s) of C. diversus ULA27 to be chromosomally encoded.
- Subjects
ANTIBIOTICS; BETA lactam antibiotics; PHYSICAL &; theoretical chemistry; COMPARATIVE studies; DRUG resistance in microorganisms; ELECTROPHORESIS; GENES; HIGH performance liquid chromatography; HYDROLASES; RESEARCH methodology; MEDICAL cooperation; RESEARCH; EVALUATION research; CITROBACTER; PHARMACODYNAMICS
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 1987, Vol 20, Issue 1, p23
- ISSN
0305-7453
- Publication type
journal article