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- Title
Transcriptional control of Clostridium autoethanogenum using CRISPRi.
- Authors
Fackler, Nicholas; Heffernan, James; Juminaga, Alex; Doser, Damien; Nagaraju, Shilpa; Gonzalez-Garcia, R Axayacatl; Simpson, Séan D; Marcellin, Esteban; Köpke, Michael
- Abstract
Gas fermentation by Clostridium autoethanogenum is a commercial process for the sustainable biomanufacturing of fuels and valuable chemicals using abundant, low-cost C1 feedstocks (CO and CO2) from sources such as inedible biomass, unsorted and nonrecyclable municipal solid waste, and industrial emissions. Efforts toward pathway engineering and elucidation of gene function in this microbe have been limited by a lack of genetic tools to control gene expression and arduous genome engineering methods. To increase the pace of progress, here we developed an inducible CRISPR interference (CRISPRi) system for C. autoethanogenum and applied that system toward transcriptional repression of genes with ostensibly crucial functions in metabolism.
- Subjects
FERMENTATION; CLOSTRIDIUM biotechnology; CRISPRS; BIOMASS gasification; BIOMASS energy industries; GENETIC engineering
- Publication
Synthetic Biology (23977000), 2021, Vol 6, Issue 1, p1
- ISSN
2397-7000
- Publication type
Article
- DOI
10.1093/synbio/ysab008