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- Title
Experimental studies of survival of anaerobic bacteria at 4°C and 22°C in two different transport systems.
- Authors
TVEDE, MICHAEL; HØIBY, NIELS
- Abstract
The survival of anaerobic bacteria on swabs in two different transport systems at 4°C and 22°C was evaluated. The transport systems were a charcoal-impregnated cotton swab in modified Stuart transport medium (MST), and a viscose swab in modified SIFF transport medium (BTM) (Bionor™). The following eight clinical strains of anaerobic bacteria were tested for quantitative recovery at 24 h, 48 h, 72 h, and 96 h; Fusobacterium necrophorum, Bacteroides melaninogenicus, Bacteroides intermedius, Peptostreptococcus anaerobius, Peptococcus magnus, Clostridium perfringens, Clostridium tetani, and Actinomyces israelii. Additionally, a mixture of Staphylococcus aureus and Escherichia coli was tested together with Bacteroides fragilis, Fusobacterium necrophorum and Clostridium perfringens. Both transport systems preserved the bacteria, but the BTM medium gave a better quantitative recovery of the bacteria than the MST in 29/64 (45%) of the experiments, whereas the opposite was the case in 15/64 (23%) of the experiments (p < 0.05). There was no significant strain-related difference between the recovery of 10 different B. fragilis strains. There was no major difference in the recovery of the anaerobes in the two systems at 4°C compared to 22°C, except for Fusobacterium necrophorum, which survived best at 4°C in the Bionor™ transport system.
- Publication
APMIS, 1992, Vol 100, Issue 7-12, p1048
- ISSN
0903-4641
- Publication type
Article
- DOI
10.1111/j.1699-0463.1992.tb04039.x