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- Title
Comparison of innate immune responses towards rhinovirus infection of primary nasal and bronchial epithelial cells.
- Authors
Alves, Marco P.; Schögler, Aline; Ebener, Simone; Vielle, Nathalie J.; Casaulta, Carmen; Jung, Andreas; Moeller, Alexander; Geiser, Thomas; Regamey, Nicolas
- Abstract
Background and objective Rhinoviruses ( RV) replicate in both upper and lower airway epithelial cells. We evaluated the possibility of using nasal epithelial cells ( NEC) as surrogate of bronchial epithelial cells ( BEC) for RV pathogenesis cell culture studies. Methods We used primary paired NEC and BEC cultures established from healthy subjects and compared the replication of RV belonging to the major ( RV16) and minor ( RV1B) group, and the cellular antiviral and proinflammatory cytokine responses towards these viruses. We related antiviral and pro-inflammatory responses of NEC isolated from CF and COPD patients with those of BEC. Results RV16 replication and major group surface receptor ( ICAM-1) expression were higher in healthy NEC compared with BEC ( P < 0.05); RV1B replication and minor group surface receptor ( LDLR) expression were similar. Healthy NEC and BEC produced similar levels of IFN-β and IFN-λ2/3 upon RV infection or after simulation with poly( IC). IL-8 production was similar between healthy NEC and BEC. IL-6 release at baseline ( P < 0.01) and upon infection with RV16 ( P < 0.05) and poly( IC) stimulation ( P < 0.05) was higher in NEC. RV1B viral load in NEC was related to RV1B viral load in BEC (r = 0.49, P = 0.01). There was a good correlation of IFN levels between NEC and BEC (r = 0.66, P = 0.0004 after RV1B infection). IL-8 production in NEC was related to IL-8 production in BEC (r = 0.48, P = 0.02 after RV1B infection). Conclusion NEC are a suitable alternative cellular system to BEC to study the pathophysiology of RV infections and particularly to investigate IFN responses induced by RV infection.
- Subjects
OBSTRUCTIVE lung diseases; CYSTIC fibrosis; EPITHELIAL cells; RHINOVIRUSES; CYTOKINES
- Publication
Respirology, 2016, Vol 21, Issue 2, p304
- ISSN
1323-7799
- Publication type
Article
- DOI
10.1111/resp.12692