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- Title
Altered Phenotype and Enhanced Antibody-Producing Ability of Peripheral B Cells in Mice with Cd19 -Driven Cre Expression.
- Authors
Zhao, Ying; Zhao, Sai; Qin, Xiao-Yuan; He, Ting-Ting; Hu, Miao-Miao; Gong, Zheng; Wang, Hong-Min; Gong, Fang-Yuan; Gao, Xiao-Ming; Wang, Jun
- Abstract
Given the importance of B lymphocytes in inflammation and immune defense against pathogens, mice transgenic for Cre under the control of Cd19 promoter (Cd19Cre/+ mice) have been widely used to specifically investigate the role of loxP-flanked genes in B cell development/function. However, impacts of expression/insertion of the Cre transgene on the phenotype and function of B cells have not been carefully studied. Here, we show that the number of marginal zone B and B1a cells was selectively reduced in Cd19Cre/+ mice, while B cell development in the bone marrow and total numbers of peripheral B cells were comparable between Cd19Cre/+ and wild type C57BL/6 mice. Notably, humoral responses to both T cell-dependent and independent antigens were significantly increased in Cd19Cre/+ mice. We speculate that these differences are mainly attributable to reduced surface CD19 levels caused by integration of the Cre-expressing cassette that inactivates one Cd19 allele. Moreover, our literature survey showed that expression of Cd19Cre/+ alone may affect the development/progression of inflammatory and anti-infectious responses. Thus, our results have important implications for the design and interpretation of results on gene functions specifically targeted in B cells in the Cd19Cre/+ mouse strain, for instance, in the context of (auto) inflammatory/infectious diseases.
- Subjects
B cells; CD19 antigen; TRANSGENIC mice; BONE marrow cells; PHENOTYPES; MICE
- Publication
Cells (2073-4409), 2022, Vol 11, Issue 4, p700
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells11040700