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- Title
Thapsigargin, Inhibitor of Sarco-Endoplasmic Ca<sup>2+</sup>-ATPase, Effectively Suppresses the Expression of S100A4 Protein in Human Breast Cancer Cell Line.
- Authors
Kotnova, A. P.; Lyanova, B. M.; Dukhanina, E. A.; Portseva, T. N.; Ilyin, Yu. V.; Georgieva, S. G.; Stepchenko, A. G.; Pankratova, E. V.
- Abstract
Thapsigargin (SERCA ATPase inhibitor) inhibited the S100A4 metastatic marker expression in MDA-MB231 breast cancer cells. We found that S100A4 gene transcription is regulated by Ca2+ signaling pathways. We found that the synthesis of S100A4 mRNA and S100A4 protein in MDA-MB231 cells was effectively suppressed by thapsigargin at a concentration of 0.4–4 μM with retaining cell viability. We assume that the change in the gene transcription in response to disturbance of Ca2+ homeostasis is directly involved in the remodeling of Ca2+ signaling pathways.
- Subjects
PROTEIN expression; CANCER cells; BREAST cancer; THAPSIGARGIN; CELL lines
- Publication
Doklady Biochemistry & Biophysics, 2019, Vol 486, Issue 1, p181
- ISSN
1607-6729
- Publication type
Article
- DOI
10.1134/S1607672919030050