We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
A dominantgdcP-specific marker derived fromMoricandia nitensused for introducing the C<sub>3</sub>-C<sub>4</sub> character fromM. nitensintoBrassicacrops.
- Authors
Zhang, C.; Xu, G.; Huang, R.; Chen, C.; Meng, J.
- Abstract
Differential localization of decarboxylation and carboxylation in association with Kranz anatomy distinguish species with C4 or C3-C4 intermediate photosynthesis from species with C3 photosynthesis.Moricandia nitenshas a C3-C4 intermediate photosynthetic mechanism and yet is closely related to C3 Brassicaspecies. In order to introduce the C3-C4 character fromM. nitensintoBrassicacrops, sesquitetraploids (MACC) were synthesized by crossingBrassica napus(AACC) and a somatic hybrid (M. nitens + B. oleracea, MMCC). Homologous fragments (2966 and 3254 bp) of P-protein gene of glycine decarboxylase (gdcP) were isolated fromM. nitensandB. napus, respectively, to develop a PCR-based marker for assisted selection. Reverse transcriptase-polymerase chain reaction (RT-PCR) of the partial encoding regions of bothgdcPs indicated that the mRNAs were expressed in the expanded leaf. The characterization of high divergence, but with large conserved island in the regulatory region of the two C3 species, implies that they may play an important role in maintaining the C3 photosynthetic pathway. Thereafter, a dominant gene-specific marker was developed within the highly divergent region for subsequent introduction of C3-C4 photosynthesis fromMoricandia nitensintoBrassicacrops.
- Subjects
BRASSICA; CROPS; PHOTOSYNTHESIS; EFFECT of light on plants; PLANT photorespiration; DECARBOXYLATION; GLYCINE; PLANT breeding
- Publication
Plant Breeding, 2004, Vol 123, Issue 5, p438
- ISSN
0179-9541
- Publication type
Article
- DOI
10.1111/j.1439-0523.2004.01030.x