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- Title
Development of molecular markers to assess the level of introgression of Populus tremula into P. alba natural populations.
- Authors
Fossati, T.; Patrignani, G.; Zapelli, I.; Sabatti, M.; Sala, F.; Castiglione, S.
- Abstract
Morphological traits traditionally adopted to discriminate between Populus alba L. and P. tremula L. have frequently led to misclassification of their spontaneous hybrid P. × canescens Sm. Moreover, they may not be of any help in cases of spontaneous backcross phenomena. These limitations can be overcome by molecular markers, which are not environmentally influenced nor subjectively assessed. In this study, the effectiveness of amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers in species and hybrid discrimination was evaluated by analysing a set of reference samples of P. alba, P. tremula and P. × canescens. Species-specific and species-indicative AFLPs, as well as diagnostic SSR alleles, were recorded in both P. alba and P. tremula reference samples. The results allowed a clear distinction between the two poplar species and their hybrid. Using these diagnostic markers, a natural population of P. alba trees sampled along the Ticino river basin in northern Italy was analysed, and P. × canescens individuals, intermingled with P. alba trees, were detected.
- Subjects
TICINO River (Switzerland &; Italy); NORTHERN Italy; POPULUS alba; EUROPEAN aspen; GENETIC polymorphisms; PLANT morphology; PLANT breeding
- Publication
Plant Breeding, 2004, Vol 123, Issue 4, p382
- ISSN
0179-9541
- Publication type
Article
- DOI
10.1111/j.1439-0523.2004.00979.x