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- Title
Application of enhanced electronegative multimodal chromatography as the primary capture step for immunoglobulin G purification.
- Authors
Wang, Yanli; Chen, Quan; Xian, Mo; Nian, Rui; Xu, Fei
- Abstract
In recent studies, electronegative multimodal chromatography with Eshmuno HCX was demonstrated to be a highly promising recovery step for direct immunoglobulin G (IgG) capture from undiluted cell culture fluid. In this study, the binding properties of HCX to IgG at different pH/salt combinations were systematically studied, and its purification performance was significantly enhanced by lowering the washing pH and conductivity after high capacity binding of IgG under its optimal conditions. A single polishing step gave an end-product with non-histone host cell protein (nh-HCP) below 1 ppm, DNA less than 1 ppb, which aggregates less than 0.5% and an overall IgG recovery of 86.2%. The whole non-affinity chromatography based two-column-step process supports direct feed loading without buffer adjustment, thus extraordinarily boosting the overall productivity and cost-savings.
- Subjects
MONOCLONAL antibodies; IMMUNOGLOBULIN G; EPITOPES; ULTRAFILTRATION; BLOOD proteins
- Publication
AMB Express, 2018, Vol 8, Issue 1, p1
- ISSN
2191-0855
- Publication type
Article
- DOI
10.1186/s13568-018-0622-3