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- Title
Identification of tumor-associated antigens by screening phage-displayed human cDNA libraries with sera from tumor patients.
- Authors
Olga Minenkova; Andrea Pucci; Emiliano Pavoni; Amedeo De Tomassi; Paola Fortugno; Nicola Gargano; Maurizio Cianfriglia; Stefano Barca; Sabino De Placido; Angelo Martignetti; Franco Felici; Riccardo Cortese; Paolo Monaci
- Abstract
Screening cDNA libraries from solid human tumors with sera of autologous patients (SEREX) has proven to be a powerful approach to identifying tumor antigens recognized by the humoral arm of the immune system. In many cases, application of this methodology has led to the discovery of novel tumor antigens as unknown gene products. We tried to improve the potency of the SEREX approach by combining it with phage-display technology. We designed a new lambda vector to express protein fragments as N-terminal fusions to the D capsid protein and generated high-complexity cDNA libraries from human breast carcinoma cell lines and solid tumors. Screening these phage-displayed libraries required limited amounts of sera from patients and efficiently identified several tumor antigens specifically reacting with sera from breast cancer patients. © 2003 Wiley-Liss, Inc.
- Subjects
DNA; ANTIGENS; IMMUNE system
- Publication
International Journal of Cancer, 2003, Vol 106, Issue 4, p534
- ISSN
0020-7136
- Publication type
Article
- DOI
10.1002/ijc.11269