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- Title
Two novel in vitro human hepatoblastoma models, HepU1 and HepU2, are highly characteristic of fetalembryonal differentiation in hepatoblastoma.
- Authors
Stefanie Scheil; Silvia Hagen; Silke Brüderlein; Ivo Leuschner; Wolfgang Behnisch; Peter Möller
- Abstract
Using comparative genomic hybridization (CGH), we present a genome-wide screening of a mixed mesenchymalepithelial hepatoblastoma, its recurrence and 2 novel hepatoblastoma cell lines raised from the ascites, 18 (HepU1) and 23 (HepU2) months after diagnosis of a hepatoblastoma in a 35-month-old boy. Both cell lines were also characterized by GTG-banding, multicolor-fluorescence in situ hybridization (M-FISH) and multicolor banding (M-Band). On the basis of CGH, we compared the cytogenetics of histologically different tumor areas of the parental tumor and its recurrence with the hepatoblastoma cell lines. We found different CGH profiles in the parental tumor rev ish enh(1q31q32,8p,12,17,20,X), dim(4q34q35,18q23)[cp] and its recurrence rev ish enh(8q24,17,Xq26q28), dim(7q11.2q21,13q34)[cp]. Although both epithelial cell lines were obtained at different times and the clonal ancestor of HepU2 had been exposed to a higher cumulative dose of chemotherapy, HepU1 and HepU2 have an identical karyotype: 48-56,XY,+Y,dup(2)(q32q34),t(3;4)(q21;q34),+8,+12,+13, +17,+t(18;19)(q21;q?),+20[cp] and identical CGH profiles: rev ish enh(2q24q33,8,12,13q,17,20), dim(4q34q35,18q22q23). In common with previously described hepatoblastoma cell lines, HepU1 and HepU2 demonstrate a gain of chromosome 20. The in situ aberrations most closely resembling that of HepU1 and HepU2 were found in areas of fetalembryonal differentiation of the primary tumor. Interestingly, both cell lines mimic this histology in their three-dimensional growth pattern in vitro. HepU1 and HepU2 are thus cytogenetically and phenotypically highly characteristic of fetalembryonal hepatoblastoma. © 2003 Wiley-Liss, Inc.
- Subjects
LIVER tumors; COMPARATIVE genomic hybridization; CELL lines; ASCITES; TUMORS in children; EPITHELIAL cells; DRUG therapy
- Publication
International Journal of Cancer, 2003, Vol 105, Issue 3, p347
- ISSN
0020-7136
- Publication type
Article
- DOI
10.1002/ijc.11082