We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Isolation of Salmonella grown poorly at 42ºC from radish sprouts.
- Authors
Fujisawa, T.; Ohashi, Y.; Yoshida, T.
- Abstract
Incubation conditions using tetrathionate broth (TT) and Rappaport-Vassiliadis broth (RV), which are typically employed as enrichment broths, were investigated for the isolation of Salmonella Brandenburg grown poorly at 42°C from radish sprouts. A small number (10² CFU/ml of broth) of Salmonella with Pseudomonas aeruginosa were injected into TT and RV; however, Salmonella colonies were absent when these broths were incubated at 42°C. No differences were observed in the detection rates of colonies on either TT or RV incubated anaerobically at 35°C and aerobically at 35°C, with the exception of a small number of Salmonella with P. aeruginosa on RV. In case of mixed culture of Salmonella Brandenburg and Escherichia coli in TT and RV at 42°C, there were samples that Salmonella colonies were not detected. In the mixed culture of Salmonella Brandenburg and radish sprouts in TT and RV, the detection rate of Salmonella was better on incubation at 35°C than that at 42°C when a large number of Salmonella Brandenburg was injected (105 or 106 CFU/ml of broth). Moreover, the detection rate at colonies was better at 35°Cthan at 42°C in the case of the injection (10² CFU/ml of broth) into RV. On the other hand, no remarkable differences were observed in the detection rates of colonies on either TT or RV incubated anaerobically at 35°C and aerobically at 35°C. These results demonstrated that the isolation of Salmonella grown poorly at 42°C from radish sprouts using a high temperature incubation was not favorable.
- Subjects
SALMONELLA; ENTEROBACTERIACEAE; FOOD pathogens; SEEDLINGS; SPROUTS
- Publication
International Food Research Journal, 2015, Vol 22, Issue 5, p2174
- ISSN
1985-4668
- Publication type
Article