We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Physiological levels of Epidermal Growth Factor in saliva stimulate cell migration of an oral epithelial cell line, HO-1-N-1.
- Authors
Ohshima, Mitsuhiro; Sato, Masashi; Ishikawa, Masako; Maeno, Masao; Otsuka, Kichibee
- Abstract
An oral epithelial cell line derived from buccal mucosa squamous cell carcinoma, HO‐1‐N‐1, was used to elucidate the role of epidermal growth factor (EGF) in saliva on wound healing of the oral mucosa. The effects of EGF on DNA synthesis, and cell migration was studied and the related signal transduction pathways examined. DNA synthesis by HO‐1‐N‐1 cells was stimulated dose‐dependently by 1–10 ng ml−1 EGF, but significantly inhibited by addition of a PI3‐K inhibitor (wortmannin), a p38 MAPK inhibitor (SB203580) or an MEKs inhibitor (PD98059). Cell migration was also accelerated by addition of 1–10 ng ml−1 EGF; however, the migration rate was decreased to 30% by adding PD98059, to 40% by adding a tyrosine kinase inhibitor (herbimycin A), and to 60% by adding wortmannin or dexamethasone. These results indicate that the physiologic concentration of EGF in saliva may stimulate proliferation and migration of oral epithelial cells for wound healing, when the oral mucosa has been injured. Furthermore, this study revealed that EGF‐stimulated signal transduction pathways for epithelial cell proliferation and cell migration are different.
- Subjects
EPIDERMAL growth factor; SALIVA
- Publication
European Journal of Oral Sciences, 2002, Vol 110, Issue 2, p130
- ISSN
0909-8836
- Publication type
Article
- DOI
10.1034/j.1600-0722.2002.11179.x