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- Title
Lysine degradation through the saccharopine pathway in bacteria: LKR and SDH in bacteria and its relationship to the plant and animal enzymes
- Authors
de Mello Serrano, Guilherme Coutinho; e Silva Figueira, Thaís Rezende; Kiyota, Eduardo; Zanata, Natalia; Arruda, Paulo
- Abstract
Abstract: Lysine degradation through the saccharopine pathway has been shown only in plants and animals. Here, we show that bacteria possess the genes encoding lysine-ketoglutarate reductase (LKR) and saccharopine dehydrogenase (SDH). In Silicibacter, the contiguous lkr and sdh genes are interspersed, in another frame, by a polypeptide of unknown function. The bacterial enzyme does not contain the 110-amino-acid interdomain (ID) that intersperses the LKR and SDH domains of the plant enzyme. The ID was found in Cyanobacteria interspersing polypeptides without similarities and activities of LKR and SDH. The LKR/SDH bifunctional polypeptide of animals and plants may have arisen from a α-proteobacterium with a configuration similar to that of Silicibacter, whereas the ID in the plant enzyme may have been inherited from Cyanobacteria.
- Subjects
LYSINE; CHEMICAL decomposition; GENETIC code; DEHYDROGENASES; POLYPEPTIDES; CYANOBACTERIA
- Publication
FEBS Letters, 2012, Vol 586, Issue 6, p905
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.02.023