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- Title
Effect of neoadjuvant chemoradiation and postoperative radiotherapy on expression of heat shock protein 70 (HSP70) in head and neck vessels.
- Authors
Tavassol, Frank; Kokemüller, Horst; Zimmerer, Rüdiger; Gellrich, Nils-Claudius; Eckardt, André; Kokemüller, Horst; Zimmerer, Rüdiger; Eckardt, André
- Abstract
<bold>Background: </bold>Preoperative radiotherapy and chemotherapy in patients with head and neck cancer result in changes to the vessels that are used to construct microsurgical anastomoses. The aim of the study was to investigate quantitative changes and HSP70 expression of irradiated neck recipient vessels and transplant vessels used for microsurgical anastomoses.<bold>Methods: </bold>Of 20 patients included in this study five patients received neoadjuvant chemoradiation, another five received conventional radiotherapy and 10 patients where treated without previous radiotherapy. During surgical procedure, vessel specimens where obtained by the surgeon. Immunhistochemical staining of HSP70 was performed and quantitative measurement and evaluation of HSP70 was carried out.<bold>Results: </bold>Conventional radiation and neoadjuvant chemoradiation revealed in a thickening of the intima layer of recipient vessels. A increased expression of HSP70 could be detected in the media layer of the recipient veins as well as in the transplant veins of patients treated with neoadjuvant chemoradiation. Radiation and chemoradiation decreased the HSP70 expression of the intima layer in recipient arteries. Conventional radiation led to a decrease of HSP70 expression in the media layer of recipient arteries.<bold>Conclusion: </bold>Our results showed that anticancer drugs can lead to a thickening of the intima layer of transplant and recipient veins and also increase the HSP70 expression in the media layer of the recipient vessels. In contrast, conventional radiation decreased the HSP70 expression in the intima layer of arteries and the media layer of recipient arteries and veins. Comparing these results with wall thickness, it was concluded, that high levels of HSP70 may prevent the intima layer of arteries and the media layer of vein from thickening.
- Subjects
RADIOTHERAPY; DRUG therapy; HEAD &; neck cancer; SURGICAL anastomosis; PROTEIN metabolism; HEAD tumors; RESEARCH; VEINS; BLOOD vessels; SMOOTH muscle; BIOLOGICAL evolution; RESEARCH methodology; ANTINEOPLASTIC agents; MEDICAL cooperation; EVALUATION research; COMPARATIVE studies; GENES; COMBINED modality therapy; NECK tumors
- Publication
Radiation Oncology, 2011, Vol 6, Issue 1, p81
- ISSN
1748-717X
- Publication type
journal article
- DOI
10.1186/1748-717X-6-81