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- Title
Quantification of Viral RNA in Multiple Pieces of Explant Liver Tissue Shows Distinct Focal Differences in Hepatitis B Infection.
- Authors
Rydell, Gustaf E; Prakash, Kasthuri; Larsson, Simon B; Skoglund, Catarina; Ringlander, Johan; Andersson, Maria E; Castedal, Maria; Norder, Heléne; Lindh, Magnus
- Abstract
Hepatitis B virus (HBV) DNA and RNA were quantified by digital PCR assays in 20-30 tissue pieces from each of 4 liver explants with cirrhosis caused by HBV. The within-patient variability of HBV RNA levels between pieces was up to a 1000-fold. Core RNA and S RNA levels were similar and correlated strongly when replication was high, supporting that transcription was from covalently closed circular DNA (cccDNA). By contrast, enhanced expression of S RNA relative to cccDNA and core RNA in patients with medium-high or low replication supports that HBV surface antigen (HBsAg) can be expressed mainly from integrated HBV DNA in such patients.
- Subjects
HEPATITIS B; CIRCULAR DNA; RNA; HEPATITIS B virus; CELL surface antigens; DNA analysis; VIRAL antigens; DNA; LIVER; HEPATITIS viruses; RESEARCH funding; CHRONIC hepatitis B; ANTIGENS
- Publication
Journal of Infectious Diseases, 2022, Vol 226, Issue 6, p1036
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiab469