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- Title
Delayed rectifier outward K<sup>+</sup> current mediates the migration of rat cerebellar granule cells stimulated by melatonin.
- Authors
Liu, Lin-Yun; Hoffman, Gloria E.; Fei, Xiao-Wei; Li, Zheng; Zhang, Zhi-Hong; Mei, Yan-Ai
- Abstract
Melatonin (MT) may work as a neuromodulator through the associated MT receptors in the central nervous system. Previously, our studies have shown that MT increased the IK current via a G protein-related pathway. In the present study, patch-clamp whole-cell recording, transwell migration assays and organotypic cerebellar slice cultures were used to examine the effect of MT on granule cell migration. MT increased the IK current amplitude and migration of granule cells. Meanwhile, TEA, the IK channel blocker, decreased the IK current and slowed the migration of granule cells. Furthermore, the effects of MT on the IK current and cell migration were not abolished by pre-incubation with P7791, a specific antagonist of MT3R, but were eliminated by the application of the MT2R antagonists K185 and 4-P-PDOT. IK current and cell migration were decreased by the application of dibutyryl cyclic AMP (dbcAMP), which was in contrast to the MT effect on the IK current and cell migration. Incubation with dbcAMP essentially blocked the MT-induced increasing effect. Moreover, incubation of isolated cell cultures in the MT-containing medium also decreased the cAMP immunoreactivity in the granule cells. It is concluded, therefore, that IK current, downstream of a cAMP transduction pathway, mediates the migration of rat cerebellar granule cells stimulated by MT.
- Subjects
MELATONIN; CELL migration; BIOLOGICAL membranes; CENTRAL nervous system; CULTURES (Biology); MEMBRANE proteins; CYCLIC adenylic acid; MICROBIAL genetics; G proteins
- Publication
Journal of Neurochemistry, 2007, Vol 102, Issue 2, p333
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/j.1471-4159.2007.04669.x