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- Title
Protective effect of buganjianyao decoction against IL-1ß- induced degeneration of endplate chondrocytes in rats via NF-κB signaling pathway.
- Authors
Xuyi Tan; Xiangzhong Qiu; Xincheng Zhang; Jie Qiu; Hao Tang; Shengchang Jiang; Fan Xue; Hao Deng
- Abstract
Purpose: To investigate the protective effect of buganjianyao decoction (BJD) against IL-1ß-induced degeneration of endplate chondrocytes in a rat model, and the underlying mechanism of action. Methods: Rat endplate chondrocytes were cultured in 6-well tissue culture plates. Two types of serum were used: normal serum and BJD-containing serum. The endplate chondrocytes were grouped as follows: blank group given 0.5% FBS, induced group treated with 0.5% volume fraction of IL-1ß (10 µg/L), normal serum groups treated with 5, 10 and 20% volume fractions of normal serum, and BJD serum groups treated with 5, 10 and 20% volume fractions of BJD-containing serum. Cell Counting Kit- 8 (CCK-8) assay was used to measure the proliferation of endplate chondrocytes. The expressions of aggrecan and matrix metalloproteinase-3(MMP-3) were determined by ELISA, while reverse transcription quantitative polymerase chain reaction (RT-qPCR) was used to assay the mRNA expressions of IγB kinasea (IKKa) and NF-κB p65. Western blotting was used measure the protein expressions of IKKa and NF-κB p65. Results: Compared with normal serum group treated with a similar volume fraction, the proliferation capacity and aggrecan expression of BJD serum group increased at 24 h and 48 h post-treatment, while expressions of MMP-3, IKKa and NF-κB p65 decreased. The effects were more pronounced in the 20% volume fraction of BJD serum group than in the other groups. Conclusion: BJD exerts protective effect against IL-1ß-induced degeneration of endplate chondrocytes via inhibition of the NF-κB signaling pathway. This finding provides an experimental basis for the potential development of BJD for the treatment of DDD.
- Subjects
REVERSE transcriptase polymerase chain reaction; NF-kappa B; MESSENGER RNA; WESTERN immunoblotting; PROTEIN expression
- Publication
Tropical Journal of Pharmaceutical Research, 2020, Vol 19, Issue 11, p2255
- ISSN
1596-5996
- Publication type
Article
- DOI
10.4314/tjpr.v19i11.2