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- Title
Interleukin 10 promotes growth and invasion of glioma cells by up-regulating KPNA 2 .
- Authors
Zhang, Zihao; Huang, Xiaoming; Li, Jie; Fan, Haitao; Yang, Fan; Zhang, Rui; Yang, Yihang; Feng, Shaobin; He, Dong; Sun, Wei; Xin, Tao
- Abstract
<bold>Objective: </bold>Glioma is one of the leading causes of death worldwide with high incidence, recurrence, and mortality. Interleukin-10 (IL-10) is a cytokine with dual function in many types of tumors. Although IL-10 is overexpressed and promotes tumor progression in human primary brain tumor, the mechanisms are largely unknown.<bold>Materials and Methods: </bold>Glioma cells were treated with different dosages of IL-10. The cell growth was detected by CCK-8, and the invasion was measured by Transwell. The relative expression of messenger RNAs was detected by quantitative real-time polymerase chain reaction.<bold>Results: </bold>We found that IL-10 treatment significantly enhanced glioma cell growth and invasion. Moreover, KPNA2 was significantly upregulated after treatment with IL-10. By performing knockdown experiments, we found that the glioma cell growth and invasion were significantly declined.<bold>Conclusions: </bold>The results indicated that knockdown of KPNA2 significantly inhibited the growth and invasion of glioma cells. Moreover, IL-10 promotes glioma progression via upregulation of KPNA2. This study will be of important significance and provides a potential target for treatment of patients with glioma.
- Subjects
CELL growth; POLYMERASE chain reaction; MESSENGER RNA; BRAIN tumors; GLIOMAS; INTERLEUKIN-10; CANCER invasiveness; KARYOPHERINS; APOPTOSIS; CELL physiology; CELL motility; GENES; INTERLEUKINS; MEMBRANE proteins; RNA; CANCER cell culture; IN vitro studies; CHEMICAL inhibitors
- Publication
Journal of Cancer Research & Therapeutics, 2019, Vol 15, Issue 4, p927
- ISSN
0973-1482
- Publication type
journal article
- DOI
10.4103/jcrt.JCRT_284_19