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- Title
Novel diagnostic biomarker for patients with Non-Hodgkin's Lymphoma by IgH gene rearrangement.
- Authors
Jian Bo; Lu Sun; Wenqing Wang; Chao Ma; Honghua Li; Yu Zhao; Bo, Jian; Sun, Lu; Wang, Wenqing; Ma, Chao; Li, Honghua; Zhao, Yu
- Abstract
<bold>Aim Of Study: </bold>Novel biomarkers for improving accuracy could be beneficial for disease monitoring and surveillance of Non-Hodgkin's lymphomas (NHL). So we explored the viability of analytical methods for identifying the rearranged immunoglobulin (Ig) H genes sequence.<bold>Materials and Methods: </bold>Next-generation sequencing (NGS) was used to sequence deoxyribonucleic acid (DNA) extracted directly from the tumor tissues of patients with NHL, and then specific rearranged DNA fragments from plasma was detected by polymerase chain reaction (PCR).<bold>Results: </bold>By parallel DNA capturing and sequencing of IgH genomic regions (IgCap), the sequence of rearranged IgH loci could be detected and precisely determined in tumor tissues of 12 patients with NHL. The circulating rearranged DNA fragments had been identified in the plasma of one patient.<bold>Conclusion: </bold>IgCap may be the favorable diagnostic method for patients with NHL in clinical.
- Subjects
LYMPHOMAS; IMMUNOGLOBULIN H genetics; BIOMARKERS; POLYMERASE chain reaction; NUCLEOTIDE sequencing; GENETICS; LYMPHOMA diagnosis; IMMUNOGLOBULINS; NUCLEOTIDES; BIOINFORMATICS; SEQUENCE analysis
- Publication
Journal of Cancer Research & Therapeutics, 2016, Vol 12, Issue 2, p903
- ISSN
0973-1482
- Publication type
journal article
- DOI
10.4103/0973-1482.157345