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- Title
Flavopiridol's antiproliferative effects in glioblastoma multiforme.
- Authors
Cobanoglu, Gozde; Turacli, Irem Dogan; Ozkan, Ayla Cihan; Ekmekci, Abdullah
- Abstract
<bold>Aim Of Study: </bold>Glioblastoma multiforme (GBM) is largely refractory to surgical operation, radiotherapy, and chemotherapy in use today. Remaining lifetime accounting for the GBM-affected patients varies between 12 and 16 months generally. The most frequently altered genes in GBM are p53, epidermal growth factor receptor, PTEN, and cyclin-dependent kinase inhibitor 2A. Our aim is to investigate the antiproliferative and apoptotic effects of flavopiridol, a cyclin-dependent kinases and specific phosphokinase inhibitor, on glioblastoma cell lines having different genetic profiles: U87MG, U118MG, and T98G.<bold>Materials and Methods: </bold>Cell viability and IC50 values were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, protein expressions were determined by Western blot and caspase activities were analyzed by activity kit.<bold>Results: </bold>Western blot analysis showed down-regulation of the cyclin D1, c-Myc, and p53 protein activities, and up-regulation of p27KIP1 activity after flavopiridol treatment. Additionally, flavopiridol diminished p-Akt protein levels generally which induces inhibition of proliferation.<bold>Conclusion: </bold>The present study demonstrated that flavopiridol did not induce caspase-3/7 activation, BIM, and BAX pro-apoptotic proteins but it leads to the expression changes of various proteins that inhibit proliferation and eternity in glioblastoma cell lines which have different genetic alterations.
- Subjects
GLIOBLASTOMA multiforme; EPIDERMAL growth factor receptors; CYCLIN-dependent kinase inhibitors; APOPTOTIC bodies; PHOSPHOKINASES; PROTEIN metabolism; ANTINEOPLASTIC agents; BIOCHEMISTRY; CELL lines; CELL physiology; FLAVONOIDS; GLIOMAS; PHENOMENOLOGY; PIPERIDINE; PROTEIN kinase inhibitors; PHARMACODYNAMICS
- Publication
Journal of Cancer Research & Therapeutics, 2016, Vol 12, Issue 2, p811
- ISSN
0973-1482
- Publication type
journal article
- DOI
10.4103/0973-1482.172132