We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
OsPFA-DSP1, a rice protein tyrosine phosphatase, negatively regulates drought stress responses in transgenic tobacco and rice plants.
- Authors
Liu, Bing; Fan, Jieqiong; Zhang, Yang; Mu, Peiqiang; Wang, Peng; Su, Jianbin; Lai, Huihuang; Li, Shaowu; Feng, Dongru; Wang, Jinfa; Wang, Hongbin
- Abstract
Dephosphorylation plays a pivotal role in regulating plant growth, development and abiotic/biotic stress responses. Here, we characterized a plant and fungi atypical dual-specificity phosphatase (PFA-DSP) subfamily member, OsPFA-DSP1, from rice. OsPFA-DSP1 was determined to be a functional protein tyrosine phosphatase (PTP) in vitro using phosphatase activity assays. Quantitative real-time PCR and GENEVESTIGATOR analysis showed that OsPFA- DSP1 mRNA was induced by drought stress. Transfection of rice protoplasts showed that OsPFA-DSP1 accumulated in both the cytoplasm and nucleus. Ectopic overexpression of OsPFA- DSP1 in tobacco increased sensitivity to drought stress and insensitivity to ABA-induced stomatal closure and inhibition of stomatal opening. Furthermore, overexpression of OsPFA- DSP1 in rice also increased sensitivity to drought stress. These results indicated that OsPFA-DSP1 is a functional PTP and may act as a negative regulator in drought stress responses.
- Subjects
PLANT growth; EFFECT of stress on plants; FUNGI; PROTEIN-tyrosine phosphatase; TOBACCO; DROUGHTS
- Publication
Plant Cell Reports, 2012, Vol 31, Issue 6, p1021
- ISSN
0721-7714
- Publication type
Article
- DOI
10.1007/s00299-011-1220-x