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- Title
Time-lapse imaging provides further evidence that planar arrangement of blastomeres is highly abnormal.
- Authors
Ebner, Thomas; Höggerl, Alexandra; Oppelt, Peter; Radler, Elisabeth; Enzelsberger, Simon-Hermann; Mayer, Richard; Petek, Erwin; Shebl, Omar; Höggerl, Alexandra; Mayer, Richard B
- Abstract
<bold>Purpose: </bold>Recently, guidelines on the annotation of dynamic human embryo monitoring recommended screening for the presence of planar blastomere arrangement at the 4-cell stage. This observational study was set up in order to analyze whether developmental kinetics of planar human embryos are different from tetrahedral ones.<bold>Methods: </bold>Therefore, embryos of 115 consecutive ICSI patients (showing 32 planar and 554 tetrahedral embryos) were cultured in a new time-lapse system (Miri TL) and their embryos were annotated for morphokinetic development and screened for irregular cleavages and morphological dysmorphisms.<bold>Results: </bold>Significantly less planar embryos reached blastocyst stage and showed worse quality as compared to regular tetrahedral embryos. The rate of bi- and/or multinucleation was also significantly higher in the affected group. Irregular cleavages, particularly embryo rolling, were more often seen in planar embryos. Morphokinetics between planar and tetrahedral were distinguishable up to 4-cell stage (t2-t4), thereafter the observed delay in planar embryos (t8) was more likely the result of a higher rate of arrested embryos in the planar group.<bold>Conclusions: </bold>Planar embryos are associated with both a significant increase in irregular cleavage as well as a delay in preimplantation development. This indicates that planar embryos are rather abnormal and should only be considered for transfer if no other embryos are available.
- Subjects
BLASTOMERES; CLEAVAGE (Embryology); PREIMPLANTATION genetic diagnosis; CHRONOPHOTOGRAPHY; MORPHOMETRICS; BLASTOCYST; EMBRYO transfer; FERTILIZATION in vitro; FETAL development; RETROSPECTIVE studies
- Publication
Archives of Gynecology & Obstetrics, 2017, Vol 296, Issue 6, p1199
- ISSN
0932-0067
- Publication type
journal article
- DOI
10.1007/s00404-017-4531-5