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- Title
基于氧化碳纳米颗粒为受体的 荧光共振能量转移体系的构建及汞离子检测应用.
- Authors
何梦媛; 尚 宁; 朱倩茹; 闫露露; 刘 畅
- Abstract
Oxidized carbon nanoparticles (OCNPs) with uniform size, good dispersion and excellent quenching performance were prepared by ultrasonic assisted nitric acid oxidation method. A fluorescence resonance energy transfer (FRET) system was constructed, with FAM as energy donors, OCNPs as energy acceptors and T-rich ssDNA as recognition molecules. The interaction between OCNPs and ssDNA brought the energy donor and acceptor in close proximity, leading to the quenching of fluorescence of Fi\M. After the addition of mercury ion ( Hg2+), the distance between the donor and acceptor was increased and the fluorescence of FA was recovered gradually, due to the formation of T-Hg2+ -T structure. Hased on the above-mentioned principle, an "'off-to-on" fluorescence sensor was established for Hg2+ detection. The linear range of mercury ion detection was 0.1~10.0 nmol/L, and the detection limit was 0. 06 nmol/L. The method showed good sensitivity and has been applied to the determination of Hg2+ in tap water and river water with a recovery of 97.4%~108.4% .
- Publication
Journal of Xinyang Normal University Natural Science Edition, 2020, Vol 33, Issue 4, p630
- ISSN
1003-0972
- Publication type
Article
- DOI
10.3969/j.issn.1003-0972.2020.04.020