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- Title
Porcine peroxisome proliferator-activated receptor γ induces transdifferentiation of myocytes into adipocytes.
- Authors
Yu, Y. H.; Liu, B. H.; Mersmann, H. J.; Ding, S. T.
- Abstract
Peroxisome proliferator-activated receptor γ2 (PPARγ) is a nuclear transcription factor that regulates adipocyte differentiation and lipogenic genes during adipogenesis. The activity of rodent PPARγ is regulated by phosphorylation of serine 112. The current experiment was designed to study the ability of porcine PPARγ to stimulate transdifferentiation of myoblasts to adipocytes by overexpressing wild-type PPARγ or mutated PPARγ (serine 112 was mutated to alanine) in mouse myoblast cells. The expression of adipogenic marker genes (adipocyte fatty acid binding protein, lipoprotein lipase, and glycerol-3 phosphate dehydrogenase) in cells stably expressing mutated porcine PPARγ was greater than in cells with wild-type PPARγ, indicating that the mutated PPARγ has greater adipogenic capability than the wild-type PPARγ. Under treatment with a ligand, both wild-type and mutant porcine PPARγ-expressing C2C12 myoblasts differentiated into adipocytes in 10 d. The expression of myogenic marker genes (myogenin, myogenic regulatory factor-4) was suppressed in cells transfected with the mutated PPARγ or wild-type PPARγ. Moreover, wild-type and mutant PPARγ were able to inhibit myogenesis without addition of a ligand. Our results suggest that porcine wild-type PPARγ and mutated PPARγ can both convert myoblast cells into adipocytes, and also that the ability to transdifferentiate was greater in cells containing the mutated PPARγ than in cells containing the wild-type PPARγ. Therefore, the existence of serine 112 in PPARγ may have a role in regulating adipocyte differentiation.
- Subjects
PORCINE somatotropin; SYNDROMES in animals; CARRIER proteins; LIPOPROTEIN lipase; AMINO acids; TRANSCRIPTION factors; MUSCLE cells; FAT cells; MYOGENESIS
- Publication
Journal of Animal Science, 2006, Vol 84, Issue 10, p2655
- ISSN
0021-8812
- Publication type
Article
- DOI
10.2527/jas.2005-645