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- Title
Evaluation of the Korean Isolate-1 Tachyzoite Antigen for Serodiagnosis of Toxoplasmosis.
- Authors
Eun-Hee Shin; Dong-Hee Kim; Aifen Lin; Jo-Woon-Yi Lee; Hyo-Jin Kim; Myoung-Hee Ahn; Jong-Yil Chai
- Abstract
To evaluate the usefulness of the Korean Isolate-1 (KI-1) antigen for serodiagnosis of toxoplasmosis, antigen profiles of KI-1 tachyzoites were analyzed in comparison with RH tachyzoites by SDS-PAGE and immunoblotting. ELISA was performed on latex agglutination (LA)-positive and negative serum samples using KI-1 and RH antigens. Immunoblotting of the KI-1 antigen showed multiple antigen bands with molecular sizes of 22-105 kDa. Among them, 1 and 6 common bands were noted against a KI-1-infected and a RH-infected human serum, respectively, which represented differences in antigenic profiles between KI-1 and RH tachyzoites. However, all 9 LA-positive human sera were found positive by ELISA, and all 12 LA-negative sera were negative by ELISA; the correlation between the ELISA titers and LA titers was high (r = 0.749). Our results suggest that tachyzoites of KI-1 may be useful for serodiagnosis of human toxoplasmosis.
- Subjects
SOUTH Korea; ANTIGENS; SERODIAGNOSIS; TOXOPLASMOSIS; TOXOPLASMA gondii
- Publication
Korean Journal of Parasitology, 2008, Vol 46, Issue 1, p45
- ISSN
0023-4001
- Publication type
Article
- DOI
10.3347/kjp.2008.46.1.45