We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Rapid identification and phylogenetic classification of diverse bacterial pathogens in a multiplexed hybridization assay targeting ribosomal RNA.
- Authors
Bhattacharyya, Roby P.; Walker, Mark; Boykin, Rich; Son, Sophie S.; Liu, Jamin; Hachey, Austin C.; Ma, Peijun; Wu, Lidan; Choi, Kyungyong; Cummins, Kaelyn C.; Benson, Maura; Skerry, Jennifer; Ryu, Hyunryul; Wong, Sharon Y.; Goldberg, Marcia B.; Han, Jongyoon; Pierce, Virginia M.; Cosimi, Lisa A.; Shoresh, Noam; Livny, Jonathan
- Abstract
Rapid bacterial identification remains a critical challenge in infectious disease diagnostics. We developed a novel molecular approach to detect and identify a wide diversity of bacterial pathogens in a single, simple assay, exploiting the conservation, abundance, and rich phylogenetic content of ribosomal RNA in a rapid fluorescent hybridization assay that requires no amplification or enzymology. Of 117 isolates from 64 species across 4 phyla, this assay identified bacteria with >89% accuracy at the species level and 100% accuracy at the family level, enabling all critical clinical distinctions. In pilot studies on primary clinical specimens, including sputum, blood cultures, and pus, bacteria from 5 different phyla were identified.
- Publication
Scientific Reports, 2019, Vol 9, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-019-40792-3