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- Title
Evaluation of α-tocopherol in virgin olive oil by a luminiscent method.
- Authors
Escuderos, M. E.; Sayago, A.; Morales, M. T.; Aparicio, R.
- Abstract
Natural fluorophores such as tocopherols are of great importance for the characterization and authentication of virgin olive oil. The band of the luminescent spectrum which is most accurately associated with the presence of α-tocopherol (380-420 nm) at λexc = 350 nm was detected and its precision was evaluated once the analytical process was optimized. A virgin olive oil, cv. Cornicabra, was spiked with several quantities of α-tocopherol, from 25 mg/Kg to 1200mg/Kg, to build a ridge regression model (adjusted-R²= 0.99) based on five wavelengths (370, 371,378, 414 and 417 nm) which are attributed to the fluorescence of this compound. The tentative model was validated (adjusted-R² = 0.87) with 8 samples of a virgin olive oil, cv. Picual, spiked with amounts of α-tocopherols ranging from 25 mg/kg to 250mg/kg. Finally, the model was successfully validated with 7 monovarietal virgin olive oils from various olive producing countries (adjusted - R² = 0.92).
- Subjects
VITAMIN E content of food; LUMINESCENT probes; OLIVE oil; REGRESSION analysis; OLIVE
- Publication
Grasas y Aceites, 2009, Vol 60, Issue 4, p336
- ISSN
0017-3495
- Publication type
Article
- DOI
10.3989/gya.108308