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- Title
Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo.
- Authors
Kutzler, M. A.; Kraynyak, K. A.; Nagle, S. J.; Parkinson, R. M.; Zharikova, D.; Chattergoon, M.; Maguire, H.; Muthumani, K.; Ugen, K.; Weiner, D. B.
- Abstract
A hurdle facing DNA vaccine development is the ability to generate strong immune responses systemically and at local immune sites. We report a novel systemically administered DNA vaccination strategy using intramuscular codelivery of CCL27 or CCL28, which elicited elevated peripheral IFN-γ and antigen-specific IgG while driving antigen-specific T-cell secretion of cytokine and antibody production in the gut-associated lymphoid tissue and lung. This strategy resulted in induction of long-lived antibody responses that neutralized influenza A/PR8/34 and protected mice from morbidity and mortality associated with a lethal intranasal viral challenge. This is the first example of the use of CCL27 and CCL28 chemokines as adjuvants to influence a DNA vaccine strategy, suggesting further examination of this approach for manipulation of vaccine-induced immunity impacting both quality and phenotype of responses.
- Subjects
PLASMIDS; CYTOPLASMIC inheritance; DNA; PREVENTIVE medicine; VACCINATION; DNA vaccines; PREVENTION of communicable diseases
- Publication
Gene Therapy, 2010, Vol 17, Issue 1, p72
- ISSN
0969-7128
- Publication type
Article
- DOI
10.1038/gt.2009.112