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- Title
Increased expression of interleukin-22 in patients with giant cell arteritis.
- Authors
Zerbini, Alessandro; Muratore, Francesco; Boiardi, Luigi; Ciccia, Francesco; Bonacini, Martina; Belloni, Lucia; Cavazza, Alberto; Cimino, Luca; Moramarco, Antonio; Alessandro, Riccardo
- Abstract
Objectives. GCA is characterized by arterial remodelling driven by inflammation. IL-22 is an attractive cytokine which acts at the crosstalk between immune and stromal cells. We hypothesized that IL-22 might be induced in GCA and might be involved in disease pathogenesis. Methods. Patients subjected to temporal artery biopsies (TABs) naive from therapy were enrolled: 27 biopsy-proven GCA, 8 biopsy-negative GCA, 21 biopsy-negative non-GCA patients. Expression of IL-22 was determined in TABs by immunohystochemistry, in plasma by ELISA, in peripheral blood mononuclear cells by real-time PCR and flow cytometry. Effects of IL-22 on viability and gene expression of primary cultures obtained from TABs were also evaluated. Results. Inflamed TABs from GCA patients showed a higher expression of IL-22 and IL-22 specific receptor subunit (IL-22R1) than non-inflamed TABs. IL-22 was expressed in infiltrating immune cells and spindle shaped cells, IL-22R1 was expressed in endothelial cells. Patients with biopsy-proven GCA showed increased levels of IL-22 in plasma than patients with biopsy-negative GCA, without GCA and healthy subjects. Peripheral blood mononuclear cells from GCA patients expressed higher IL-22 transcript than healthy subjects. After stimulation in vitro with phorbol 12-myristate 13-acetate and ionomycin, the frequencies of Th22 and IL-22+ CD4+ lymphocytes were similar between patients with and without GCA. Treatment with IL-22 of primary cultures obtained from TABs increased cell viability under stress conditions and expression of B-cell activating factor. Conclusion. IL-22 is increased in patients with GCA and affects viability and gene expression of arterial cells, supporting a potential role in disease pathogenesis.
- Subjects
GIANT cell arteritis diagnosis; ACETIC acid; BIOPSY; CELL culture; CELL receptors; ENZYME-linked immunosorbent assay; EPITHELIAL cells; FATTY acids; FLOW cytometry; GENE expression; IMMUNOHISTOCHEMISTRY; INTERLEUKINS; POLYMERASE chain reaction; T cells; TUMOR necrosis factors; UNSATURATED fatty acids; MONONUCLEAR leukocytes
- Publication
Rheumatology, 2018, Vol 57, Issue 1, p64
- ISSN
1462-0324
- Publication type
Article
- DOI
10.1093/rheumatology/kex334