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- Title
Pregnancy associates with alterations to the host and microbial proteome in vaginal mucosa.
- Authors
Farr Zuend, Christina; Tobin, Nicole H.; Vera, Trisha; Kotyrba, Lani; Noël‐Romas, Laura; Birse, Kenzie; Mutch, Sarah; Li, Fan; Lee, David; McCorrister, Stuart; Westmacott, Garrett; Aldrovandi, Grace M.; Burgener, Adam D.
- Abstract
Problem: Pregnant women are at increased risk of HIV acquisition, but the biological mechanisms contributing to this observation are not well understood. Method of Study: Here, we assessed host immune and microbiome differences in the vaginal mucosa of healthy pregnant and non‐pregnant women using a metaproteomics approach. Cervicovaginal lavage (CVL) samples were collected from 23 pregnant and 25 non‐pregnant women. Results: Mass spectrometry analysis of CVL identified 550 human proteins and 376 bacterial proteins from 11 genera. Host proteome analysis indicated 56 human proteins (10%) were differentially abundant (P <.05) between pregnant and non‐pregnant women, including proteins involved in angiogenesis (P = 3.36E‐3), cell movement of phagocytes (P = 1.34E‐6), and permeability of blood vessels (P = 1.27E‐4). The major bacterial genera identified were Lactobacillus, Gardnerella, Prevotella, Megasphaera, and Atopobium. Pregnant women had higher levels of Lactobacillus species (P =.017) compared with non‐pregnant women. Functional pathway analysis indicated that pregnancy associated with changes to bacterial metabolic pathway involved in energy metabolism, which were increased in pregnant women (P =.035). Conclusion: Overall, pregnant women showed differences in the cervicovaginal proteome and microbiome that may be important for HIV infection risk.
- Subjects
BACTERIAL proteins; PREGNANT women; MASS analysis (Spectrometry); PROTEOMICS; CELL motility
- Publication
American Journal of Reproductive Immunology, 2020, Vol 83, Issue 6, p1
- ISSN
1046-7408
- Publication type
Article
- DOI
10.1111/aji.13235