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- Title
Polymerase chain reaction banding patterns of the 5S rDNA gene as a diagnostic tool for the discrimination of South American mullets of the genus Mugil.
- Authors
Rodrigues-Filho, Luis Fernando S; da Cunha, Divino Bruno; Vallinoto, Marcelo; Schneider, Horacio; Sampaio, Iracilda; Fraga, Elmary
- Abstract
The mullets of the family Mugilidae, and in particular the species of the genus Mugil, are characterized by highly conserved morphological features, which hinder taxonomic classi¢cation considerably. Given this, the present study aimed to evaluate the efficiency of the 5S rDNA gene ^ a rapid and low cost genotyping marker ^ for the discrimination of Mugil species.Variations in the banding pattern permitted the reliable di!erentiation of four species ^ Mugil curema, Mugil incilis, Mugil sp. and Mugil hospes ^ although those of three others (Mugil liza, Mugil platanus and Mugil cephalus) were identical. This suggests that these three forms have diverged very recently, on a time scale that has limitedchanges in the length of the fragments of the 5S rDNA gene. The results of the study con¢rm the e⁄ciency of this marker as a diagnostic tool for the identi¢cation of Mugil species, and its usefulness for population management and pisciculture, an important economic activity involving this group.
- Subjects
POLYMERASE chain reaction; RECOMBINANT DNA; GRAY mullets; ANIMAL morphology; IDENTIFICATION of fishes
- Publication
Aquaculture Research, 2011, Vol 42, Issue 8, p1117
- ISSN
1355-557X
- Publication type
Article
- DOI
10.1111/j.1365-2109.2010.02698.x