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- Title
Self-Assembly of Functional Nucleic Acid-Based Colorimetric Competition Assay for the Detection of Immunoglobulin E.
- Authors
Lin, Xuexia; Yu, Caiyun; Lin, Honggui; Wang, Cui; Su, Jianlong; Cheng, Jie; Kankala, Ranjith Kumar; Zhou, Shu-Feng
- Abstract
In this work, we have developed a simple and rapid colorimetric assay for the detection of immunoglobulin E (IgE) using functional nucleic acids (FNAs) and a solid-phase competition enzyme-linked immunosorbent assay (ELISA). The FNAs including aptamer of recombinant IgE, G-quadruplex and its complementary fragments were immobilized on 96-well microplates to achieve recognition and detection of IgE in biological samples. The G-quadruplex DNAzyme catalyzed 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS)-hemin-H2O2 system was used to improve the sensitivity of colorimetric assay. In the presence of IgE, the hairpin structure and G-quadruplex would be destroyed, resulting in the inactivation of DNAzyme and subsequent reduction of its absorbance. This cost-effective approach detected IgE in the linear range from 5.0 pg/mL to 500 ng/mL, with the limit of detection (LOD) of 2.0 pg/mL, under optimal conditions. Moreover, the developed method was successfully applied to the rapid detection of IgE in human urine, indicating a great potentiality of this approach in clinical diagnosis and other biomedical applications.
- Subjects
ENZYME-linked immunosorbent assay; QUADRUPLEX nucleic acids; IMMUNOGLOBULIN E; COLORIMETRIC analysis; MOLECULAR self-assembly; BIOSENSORS
- Publication
Sensors (14248220), 2019, Vol 19, Issue 10, p2224
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s19102224