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- Title
Genotyping Single Nucleotide Polymorphisms Using Different Molecular Beacon Multiplexed within a Suspended Core Optical Fiber.
- Authors
Linh Viet Nguyen; Giannetti, Sara; Warren-Smith, Stephen; Cooper, Alan; Selleri, Stefano; Cucinotta, Annamaria; Monro, Tanya
- Abstract
We report a novel approach to genotyping single nucleotide polymorphisms (SNPs) using molecular beacons in conjunction with a suspended core optical fiber (SCF). Target DNA sequences corresponding to the wild- or mutant-type have been accurately recognized by immobilizing two different molecular beacons on the core of a SCF. The two molecular beacons differ by one base in the loop-probe and utilize different fluorescent indicators. Single-color fluorescence enhancement was obtained when the immobilized SCFs were filled with a solution containing either wild-type or mutant-type sequence (homozygous sample), while filling the immobilized SCF with solution containing both wild- and mutant-type sequences resulted in dual-color fluorescence enhancement, indicating a heterozygous sample. The genotyping was realized amplification-free and with ultra low-volume for the required DNA solution (nano-liter). This is, to our knowledge, the first genotyping device based on the combination of optical fiber and molecular beacons.
- Subjects
GENETIC polymorphism research; OPTICAL fibers; NUCLEOTIDE sequencing; FLUORESCENCE; MOLECULES; SINGLE nucleotide polymorphisms
- Publication
Sensors (14248220), 2014, Vol 14, Issue 8, p14488
- ISSN
1424-8220
- Publication type
Article
- DOI
10.3390/s140814488