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- Title
A Cancer-Related microRNA Signature Shows Biomarker Utility in Multiple Myeloma.
- Authors
Papanota, Aristea-Maria; Karousi, Paraskevi; Kontos, Christos K.; Artemaki, Pinelopi I.; Liacos, Christine-Ivy; Papadimitriou, Maria-Alexandra; Bagratuni, Tina; Eleutherakis-Papaiakovou, Evangelos; Malandrakis, Panagiotis; Ntanasis-Stathopoulos, Ioannis; Gavriatopoulou, Maria; Kastritis, Efstathios; Avgeris, Margaritis; Dimopoulos, Meletios-Athanasios; Scorilas, Andreas; Terpos, Evangelos
- Abstract
Multiple myeloma (MM) is the second most common hematological malignancy, arising from terminally differentiated B cells, namely plasma cells. miRNAs are small non-coding RNAs that participate in the post-transcriptional regulation of gene expression. In this study, we investigated the role of nine miRNAs in MM. CD138+ plasma cells were selected from bone marrow aspirates from MM and smoldering MM (sMM) patients. Total RNA was extracted and in vitro polyadenylated. Next, first-strand cDNA synthesis was performed using an oligo-dT–adapter primer. For the relative quantification of the investigated miRNAs, an in-house real-time quantitative PCR (qPCR) assay was developed. A functional in silico analysis of the miRNAs was also performed. miR-16-5p and miR-155-5p expression was significantly lower in the CD138+ plasma cells of MM patients than in those of sMM patients. Furthermore, lower levels of miR-15a-5p, miR-16-5p, and miR-222-3p were observed in the CD138+ plasma cells of MM patients with osteolytic bone lesions, compared to those without. miR-125b-5p was also overexpressed in the CD138+ plasma cells of MM patients with bone disease that presented with skeletal-related events (SREs). Furthermore, lower levels of miR-223-3p were associated with significantly worse overall survival in MM patients. In conclusion, we propose a miRNA signature with putative clinical utility in MM.
- Subjects
MULTIPLE myeloma; OVERALL survival; NON-coding RNA; PLASMA cells; MICRORNA; GENETIC regulation
- Publication
International Journal of Molecular Sciences, 2021, Vol 22, Issue 23, p13144
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms222313144