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- Title
Phosphoproteomics Meets Chemical Genetics: Approaches for Global Mapping and Deciphering the Phosphoproteome.
- Authors
Jurcik, Jan; Sivakova, Barbara; Cipakova, Ingrid; Selicky, Tomas; Stupenova, Erika; Jurcik, Matus; Osadska, Michaela; Barath, Peter; Cipak, Lubos
- Abstract
Protein kinases are important enzymes involved in the regulation of various cellular processes. To function properly, each protein kinase phosphorylates only a limited number of proteins among the thousands present in the cell. This provides a rapid and dynamic regulatory mechanism that controls biological functions of the proteins. Despite the importance of protein kinases, most of their substrates remain unknown. Recently, the advances in the fields of protein engineering, chemical genetics, and mass spectrometry have boosted studies on identification of bona fide substrates of protein kinases. Among the various methods in protein kinase specific substrate identification, genetically engineered protein kinases and quantitative phosphoproteomics have become promising tools. Herein, we review the current advances in the field of chemical genetics in analog-sensitive protein kinase mutants and highlight selected strategies for identifying protein kinase substrates and studying the dynamic nature of protein phosphorylation.
- Subjects
RECOMBINANT proteins; PROTEIN kinases; PROTEIN genetics; MUTANT proteins; PROTEOMICS; ENZYME regulation; BIOCHEMICAL genetics
- Publication
International Journal of Molecular Sciences, 2020, Vol 21, Issue 20, p7637
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms21207637