We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
New Aspects Towards a Molecular Understanding of the Allicin Immunostimulatory Mechanism via Colec12, MARCO, and SCARB1 Receptors.
- Authors
Toma, Vlad Al.; Tigu, Adrian Bogdan; Farcaș, Anca D.; Sevastre, Bogdan; Taulescu, Marian; Gherman, Ana Maria Raluca; Roman, Ioana; Fischer-Fodor, Eva; Pârvu, Marcel
- Abstract
The allicin pleiotropic effects, which include anti-inflammatory, anti-oxidant, anti-tumoral, and antibacterial actions, were well demonstrated and correlated with various molecular pathways. The immunostimulatory mechanism of allicin has not been elucidated; however, there is a possible cytokine stimulation from immunoglobulin release caused by allicin. In this study, when Wistar female rats and CD19+ lymphocytes were treated with three different doses of allicin, immunoglobulins, glutathione, and oxidative stress markers were assayed. Molecular docking was performed between S-allylmercaptoglutathione (GSSA)—a circulating form of allicin in in vivo systems formed by the allicin interaction with glutathione (GSH)—and scavenger receptors class A and B from macrophages, as well as CD19+ B lymphocytes. Our data demonstrated a humoral immunostimulatory effect of allicin in rats and direct stimulation of B lymphocytes by S-allyl-mercapto-glutathione, both correlated with decreased catalase (CAT) activity. The molecular docking revealed that S-allyl-mercapto-glutathione interacting with Colec12, MARCO (class A), and SCARB1 (class B) scavenger receptors in in vitro tests demonstrates a direct stimulation of immunoglobulin secretion by GSSA in CD19+ B lymphocytes. These data collectively indicate that GSSA stimulates immunoglobulin secretion by binding on scavenger receptors class B type 1 (SCARB1) from CD19+ B lymphocytes.
- Subjects
MOLECULAR docking; OXIDATIVE stress; IMMUNOGLOBULINS; LYMPHOCYTES; GLUTATHIONE
- Publication
International Journal of Molecular Sciences, 2019, Vol 20, Issue 15, p3627
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms20153627