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- Title
Dietary Phenethyl Isothiocyanate Protects Mice from Colitis Associated Colon Cancer.
- Authors
Yi Liu; Dey, Moul
- Abstract
We have previously reported alleviation of dextran sodium sulfate (DSS)-induced ulcerative colitis signs in phenethyl isothiocyanate (PEITC)-treated mice. Here we investigated chemoprotective activities of PEITC in mice with Azoxymethane-DSS induced colitis associated colon carcinogenesis. We also examined the molecular mediators associated with the PEITC effects using relevant cell lines. A 0.12% PEITC-enriched mouse-diet reduced mucosal and submucosal inflammation as well as glandular atypia by 12% and the frequency of adenocarcinoma by 17% with a concomitant improvement in overall disease activity indices compared to the diseased control group. Lipopolysaccharide-induced in vitro up-regulation of key mediators of inflammation, immune response, apoptosis, and cell proliferation were attenuated by 10 μM PEITC. Three of these mediators showed concentration-dependent reduction in respective mRNAs. Furthermore, PEITC inhibited Nuclear factor kappa B1 (NFκB1) proteins in a concentration-dependent manner. The NFkB1 mRNA expression inversely correlated (r = -0.940, p = 0.013) with tri-methylation of lysine 27 on histone 3 near its promoter region in a time-dependent manner. These results indicate that PEITC may slow down the development of colon carcinogenesis in an inflammatory intestinal setting which is potentially associated with epigenetic modulation of NFkB1 signaling.
- Subjects
ISOTHIOCYANATES; LABORATORY mice; COLITIS diagnosis; COLON cancer treatment; DEXTRAN
- Publication
International Journal of Molecular Sciences, 2017, Vol 18, Issue 9, p1908
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms18091908