We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Immunogenicity of recombinant Lactobacillus plantarum NC8 expressing goose parvovirus VP2 gene in BALB/c mice.
- Authors
Yu-Ying Liu; Wen-Tao Yang; Shao-Hua Shi; Ya-Jie Li; Liang Zhao; Chun-Wei Shi; Fang-Yu Zhou; Yan-Long Jiang; Jing-Tao Hu; Wei Gu; Gui-Lian Yang; Chun-Feng Wang
- Abstract
Goose parvovirus (GPV) continues to be a threat to goose farms and has significant economic effects on the production of geese. Current commercially available vaccines only rarely prevent GPV infection. In our study, Lactobacillus (L.) plantarum NC8 was selected as a vector to express the VP2 gene of GPV and recombinant L. plantarum pSIP409-VP2/NC8 was successfully constructed. The molecular weight of the expressed recombinant protein was approximately 70 kDa. Mice were immunized with a 2 x 109 colony-forming unit/200 μL dose of the recombinant L. plantarum strain and the ratios and numbers of CD11c+, CD3+CD4+, CD3+CD8+ and interferon gamma- and tumor necrosis factor alpha-expressing spleen lymphocytes in the pSIP409-VP2/NC8 group were higher than those in the control groups. In addition, we assessed the capacity of L. plantarum SIP409-VP2/NC8 to induce secretory IgA production. We conclude that administered pSIP409-VP2/NC8 leads to relatively extensive cellular responses. This study provides information on GPV infection and offers a clear framework of options available for GPV control strategies.
- Subjects
LACTOBACILLUS plantarum; GEESE; PARVOVIRUS diseases; MOLECULAR weights; IMMUNIZATION
- Publication
Journal of Veterinary Science, 2017, Vol 18, Issue 2, p159
- ISSN
1229-845X
- Publication type
Article
- DOI
10.4142/jvs.2017.18.2.159