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- Title
Optimum conditions for sterilization of Geobacillus stearothermophilus spores using plasma-based ion implantation.
- Authors
Motoko Hiyama; Koji Kakugawa; Aya Yakushiji; Kenichi Watanabe; Ryota Matsuda; Yoshinobu Tsuchiya; Takeshi Tanaka
- Abstract
In recent years, substantial research into low-temperature sterilization techniques has been conducted. Plasma-based ion implantation (PBII) is a low-temperature, cost-effective technique for sterilizing medical equipment, containers, and foods. We sought to optimize the seterilization conditions for Geobacillus stearothermophilus spores, which are highly heat tolerant, using a PBII apparatus. G. stearothermophilus spore survivors decreased from 107 to 101 cfu/mL under the following conditions: O2 atmosphere, 1 kHz frequency, 50 μs delay time, 20 µs pulse width, -12 kV pulse voltage, 240 V, RF power, and a 10 min exposure time. We were able, therefore, to achieve "6D" sterilization using low-temperature sterilization conditions.
- Subjects
GEOBACILLUS stearothermophilus; ION implantation; IRRADIATION; RADIO frequency; INDUCTIVELY coupled plasma spectrometry
- Publication
Electrotechnica & Electronica (E+E), 2018, Vol 53, Issue 7/8, p199
- ISSN
0861-4717
- Publication type
Article