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- Title
Yoğun Bakımda Sedasyon Amaçlı Kullanılan ID İlaçların Biyokimyasal Markerlara Olan İnterferans Etkisinin Deneysel Araştırılması.
- Authors
Büyükfırat, Evren; Gönel, Ataman; Karahan, Mahmut Alp; Altay, Nuray; Erol, Mehmet Kenan; Pehlivan, Başak; Atlas, Ahmet
- Abstract
Objective: Due to drug interference, incorrect results can be obtained in laboratory tests. The aim of this study is to investigate the interference effects of ketamine, thiopental sodium, propofol, midazolam and dexmedetomidine used in intensive care for sedation on routine biochemical tests measured by immunoassay and spectrophotometric methods. Methods: 20 μL from 5 different drug solutions was added to 180 μL control solution and mixed using vortex for 5 seconds. Biochemical tests were measured from the mixture using spectrophotometric and immunoassay methods. Each measurement was made separately for 5 different drugs and repeated for 3 times, and 20 μL of distilled water was used as the control material, and their average values were obtained. Average value was accepted as the target value, and deviation rates were determined with % bias. Results: After application of all drugs, negative interference occurred at a rate of 13.51% to 30.81% in creatine kinase MB (CK-MB) test; positive interference at a rate of 11.67% to 68.33% in troponin I test in propofol, thiopental and dexmedetomidine. In N-terminal pro Brain Natriuretic Peptide (NT-proBNP) test, positive interference developed between 16.42-37.20% in propofol, ketamine, midazolam and dexmedetomidine, and negative interference with thiopental at a rate of 85.27%. The highest interference was observed with thiopental in free thyroiodothyronine (FT3), free thyroxine (FT4), thyroid stimulating hormone (TSH) tests, while the lowest interference was found with dexmedetomidine. In C-reactive protein (CRP) test, the highest negative interference (96.43%) was detected in thiopental, while the lowest negative interference (10.71%) with dexmedetomine. Interference was less common in spectrophotometric tests, while it was most frequently detected in sodium and chlorine tests. Conclusion: In the analytical phase of the drugs used for sedation, a significant interference occurred in immunoassay tests. Less deviation was detected in spectrophotometric method performed with biochemical markers. Change in test results may be the result of erroneous measurement due to drug use other than diseases. Clinicians should evaluate the possibility of incorrect measurement due to drug-kit interaction in questionable test resuls.
- Subjects
CRITICAL care medicine; CONSCIOUS sedation; BIOMARKERS; PROPOFOL; MIDAZOLAM; DEXMEDETOMIDINE
- Publication
Journal of Anesthesia / Anestezi Dergisi (JARSS), 2020, Vol 28, Issue 4, p293
- ISSN
1300-0578
- Publication type
Article
- DOI
10.5222/jarss.2020.37232