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- Title
Effects of Long-term Application of Cattle Manure on Antibiotic Resistome in Cinnamon Soil.
- Authors
Liu Zhiping; Xie Wenyan; Yang Zhenxing; Hu Xuechun; Zhang Pengfei; Zhou Huaiping
- Abstract
In order to explore the effects of long-term application of cattle manure on soil antibiotic resistome, five fertilization treatments were selected from the long-term location experiment in the cinnamon soil of Shouyang, Shanxi Province, i.e., CK (No fertilization), N2 P1M1(N: 120 kg·hm-2, P: 37.5 kg·hm-2 ; Cow dung: 22 500 kg· hm-2 ), N4 P2M2(N: 240 kg·hm-2 ; P: 75 kg·hm-2 ; Cow dung: 45 000 kg·hm-2 ), N3 P2M3(N: 180 kg·hm-2 ; P: 75 kg·hm-2 ; Cow dung: 67 500 kg·hm-2 ) and N0 P0M6(Cow dung: 135 000 kg·hm-2 ). Soil samples of surface layer (0 -20 cm) at the middle growth stage of maize were collected. A total of 384 antibiotics resistance genes (ARGs), mobile genetic elements and related taxonomic genes in soil were detected by high-throughput quantitative realtime polymerase chain reaction (HT-qPCR), and their diversity and correlation with soil physical and chemical factors were analyzed. The results showed that with the increase of cow manure application amount, the diversity of ARGs related genes increased gradually. There were 64 shared ARGs related gene subtypes in the five treatments, and 21 specific ARGs related gene subtypes in treatment N0 P0M6, which was significantly higher than other treatments. The highest abundance of ARGs related gene subtypes in all treatments were AAC(6' )- Im, ANT(4' )- Ia, aadA5, AAC(3)- Viaand copA. The highest abundance of ARGs types in all treatments were aminoglycoside, multi- drug, tetracycline, fluoroquinolone and macrolide-lincosamide-streptogramin B (MLSB). The β-diversity of ARGs related gene subtypes was significantly different among different treatments. Redundancy analysis showed that pH, total nitrogen and organic matter were the most important environmental factors affecting the difference of ARGs between different treatments. In conclusion, although the returning livestock and poultry manure to the field can improve soil fertility and promote crop growth, it can also cause the dispersal of ARGs in the soil ecosystem. Therefore, in the future of agricultural production, environmental and ecological benefits should be taken into comprehensive consideration to strengthen the harmless treatment of manure, so as to control the environmental risk of ARGs.
- Subjects
SHANXI Sheng (China); CATTLE manure; MOBILE genetic elements; POULTRY manure; CINNAMON; SOILS; SOIL fertility
- Publication
Asian Journals of Ecotoxicology, 2023, Vol 18, Issue 1, p78
- ISSN
1673-5897
- Publication type
Article
- DOI
10.7524/AJE.1673-5897.20220608002