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- Title
A Monocentric Cohort of Obstetric Seronegative Anti-Phospholipid Syndrome.
- Authors
Truglia, Simona; Capozzi, Antonella; Mancuso, Silvia; Recalchi, Serena; Spinelli, Francesca Romana; Perricone, Carlo; De Carolis, Caterina; Manganelli, Valeria; Riitano, Gloria; Garofalo, Tina; Longo, Agostina; De Carolis, Sara; Alessandri, Cristiano; Misasi, Roberta; Valesini, Guido; Sorice, Maurizio; Conti, Fabrizio
- Abstract
The present study was conducted to diagnose obstetric anti-phospholipid syndrome (OAPS) in patients with clinical signs suggestive of anti-phospholipid syndrome (APS), but persistently negative for conventional anti-phospholipid antibodies (aPL). Sera from 61 obstetrical seronegative APS (SN-APS) patients were analyzed for anti-cardiolipin antibodies (aCL) using thin-layer chromatography (TLC)-immunostaining, for anticardiolipin/vimentin antibodies (aCL/Vim), anti-phosphatidylserine/prothrombin antibodies, IgA anti-ß2glycoprotein I antibodies (aß2GPI), and IgA aCL antibodies by enzymelinked immunosorbent assay. Taken together, our findings show that in 50 out of 61 SN-APS (81.9%) at least one aPL/cofactor antibody was detected using the assays under test. Results revealed that 76% of SN-APS patients resulted positive for aCL by TLC-immunostaining, 54% for aCL/Vim, 12% for aPS/PT, 4% for IgA aß2GPI, and 2% for IgA aCL. Thirty-five out of 61 patients were followed up and the tests were repeated on two occasions, at least 12 weeks apart. Twenty-six out of 35 SN-APS (74.3%) were positive at least one non-conventional test; only 2 patients (5.7%) did not confirm the positivity to the second test. These findings suggest that non-conventional tests, mainly aCL/Vim and aCL detected by TLC-immunostaining, seem to be the most sensitive approaches for finding out aPL in patients with obstetric SN-APS. The use of these tests can be useful for accurate and timely diagnosis of patients with obstetrical APS who are negative for conventional laboratory criteria markers.
- Subjects
CARDIOLIPIN; PROTHROMBIN; THIN layer chromatography
- Publication
Frontiers in Immunology, 2018, p1
- ISSN
1664-3224
- Publication type
Article
- DOI
10.3389/fimmu.2018.01678