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- Title
Disperse Orange 3 as a resonance Raman probe for measuring membrane order.
- Authors
Numakura, Yuki; Miura, Takashi
- Abstract
Resonance Raman spectra of azobenzene derivatives were examined in the presence of lipid membranes to find a probe that can distinguish different membrane phases. The NO 2 symmetric stretching band of 4-(4-nitrophenylazo)aniline, also known as Disperse Orange 3 (DO3), is downshifted by about 4 cm −1 on the phase transition of phosphatidylcholine membranes from the liquid crystalline to the gel phase. A comparable downshift also occurs when DO3 is bound to cholesterol-containing membranes in the liquid-ordered phase. Our results demonstrate that Raman spectrum of DO3 is a unique tool for measuring the molecular order of lipids in membranes.
- Subjects
RESONANCE; MOLECULAR probes; RAMAN spectra; AZOBENZENE derivatives; BILAYER lipid membranes
- Publication
FEBS Open Bio, 2015, Vol 5, p859
- ISSN
2211-5463
- Publication type
Article
- DOI
10.1016/j.fob.2015.10.008