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- Title
The role of Cysteine 227 in subcellular localization, water permeability, and multimerization of aquaporin‐11.
- Authors
Takahashi, Saki; Muta, Kanako; Sonoda, Hiroko; Kato, Ayaka; Abdeen, Ahmed; Ikeda, Masahiro
- Abstract
Aquaporin‐11 (AQP11) is the latest member of the mammalian water channel protein family to be described. Recent in vivo studies have shown that mutation at Cys227 causes renal failure. However the importance of Cys227 for the molecular function of AQP11 is largely unknown. In this study, we examined the subcellular localization, water permeability, and multimerization of AQP11 with a mutation at Cys227. Interestingly, cells expressing the mutants had significantly higher osmotic water permeability. In contrast, the mutation lowered the cell surface expression and multimerization levels. Our observations suggest that Cys227 is crucial for the proper molecular function of AQP11.
- Subjects
AQUAPORINS; GENETIC mutation; KIDNEY failure; CELL membranes; MEMBRANE proteins; DISEASE risk factors
- Publication
FEBS Open Bio, 2014, Vol 4, Issue 1, p315
- ISSN
2211-5463
- Publication type
Article
- DOI
10.1016/j.fob.2014.03.005