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- Title
A y<sup>+</sup>LAT-1 mutant protein interferes with y<sup>+</sup>LAT-2 activity: implications for the molecular pathogenesis of lysinuric protein intolerance.
- Authors
Sperandeo, Maria P.; Paladino, Simona; Maiuri, Luigi; Maroupulos, George D.; Zurzolo, Chiara; Taglialatela, Maurizio; Andria, Generoso; Sebastio, Gianfranco
- Abstract
Lysinuric protein intolerance (LPI) is an inherited aminoaciduria caused by defective cationic amino acid (CAA) transport at the basolateral membrane of epithelial cells in the intestine and kidney. The SLC7A7 gene, mutated in LPI, encodes the y+LAT-1 protein, which is the light subunit of the heterodimeric CAA transporter in which 4F2hc is the heavy chain subunit. Co-expression of 4F2hc and y+LAT-1 induces the y+L activity. This activity is also exerted by another complex composed of 4F2hc and y+LAT-2, the latter encoded by the SLC7A6 gene and more ubiquitously expressed than SLC7A7. On the basis of both the pattern of expression and the transport activity, y+LAT-2 might compensate for CAA transport when y+LAT-1 is defective. By expression in Xenopus laevis oocytes and mammalian cells, we functionally analysed two SLC7A7 mutants, E36del and F152L, respectively, the former displaying a partial dominant-negative effect. The results of the present study provide further insight into the molecular pathogenesis of LPI: a putative multiheteromeric structure of both[4F2hc/y+LAT-1]and[4F2hc/y+LAT-2], and the interference between y+LAT-1 and y+LAT-2 proteins. This interference can explain why the compensatory mechanism, that is, an increased expression of SLC7A6 as seen in lymphoblasts from LPI patients, may not be sufficient to restore the y+L system activity.European Journal of Human Genetics (2005) 13, 628-634. doi:10.1038/sj.ejhg.5201376 Published online 9 March 2005
- Subjects
MOLECULAR pathology; LYSINE; AMINO acids; GENETIC mutation; EPITHELIAL cells; PROTEINS
- Publication
European Journal of Human Genetics, 2005, Vol 13, Issue 5, p628
- ISSN
1018-4813
- Publication type
Article
- DOI
10.1038/sj.ejhg.5201376