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- Title
Phosphonated Near-Infrared Fluorophores for Biomedical Imaging of Bone.
- Authors
Hyun, Hoon; Wada, Hideyuki; Bao, Kai; Gravier, Julien; Yadav, Yogesh; Laramie, Matt; Henary, Maged; Frangioni, John V.; Choi, Hak Soo
- Abstract
The conventional method for creating targeted contrast agents is to conjugate separate targeting and fluorophore domains. A new strategy is based on the incorporation of targeting moieties into the non-delocalized structure of pentamethine and heptamethine indocyanines. Using the known affinity of phosphonates for bone minerals in a model system, two families of bifunctional molecules that target bone without requiring a traditional bisphosphonate are synthesized. With peak fluorescence emissions at approximately 700 or 800 nm, these molecules can be used for fluorescence-assisted resection and exploration (FLARE) dual-channel imaging. Longitudinal FLARE studies in mice demonstrate that phosphonated near-infrared fluorophores remain stable in bone for over five weeks, and histological analysis confirms their incorporation into the bone matrix. Taken together, a new strategy for creating ultra-compact, targeted near-infrared fluorophores for various bioimaging applications is described.
- Subjects
FLUOROPHORES; IMAGING compatibility of biomedical materials; PHARMACEUTICAL chemistry; CONTRAST media; ANTIBODY-enzyme conjugates
- Publication
Angewandte Chemie International Edition, 2014, Vol 53, Issue 40, p10668
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201404930