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- Title
The Structure of a Transient Complex of a Nonribosomal Peptide Synthetase and a Cytochrome P450 Monooxygenase.
- Authors
Haslinger, Kristina; Brieke, Clara; Uhlmann, Stefanie; Sieverling, Lina; Süssmuth, Roderich D.; Cryle, Max J.
- Abstract
Studying the interplay between nonribosomal peptide synthetases (NRPS), a major source of secondary metabolites, and crucial external modifying enzymes is a challenging task since the interactions involved are often transient in nature. By applying a range of synthetic inhibitor-type compounds, a stabilized complex appropriate for structural analysis was generated for such a tailoring enzyme and an NRPS domain. The complex studied comprises an NRPS peptidyl carrier protein (PCP) domain bound to the Cytochrome P450 enzyme that is crucial for the provision of β-hydroxylated amino acid precursors in the biosynthesis of the cyclic depsipeptide skyllamycin. The structure reveals that complex formation is governed by hydrophobic interactions, the presence of which can be controlled through minor alterations in PCP structure that enable selectivity amongst multiple highly similar PCP domains.
- Subjects
PEPTIDE synthesis; CYTOCHROMES; MONOOXYGENASES; NONRIBOSOMAL peptide synthetases; AMINO acids; BIOSYNTHESIS
- Publication
Angewandte Chemie International Edition, 2014, Vol 53, Issue 32, p8518
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201404977